Sequence-Ready Map

A physical map of a genome that provides the necessary templates for large-scale sequencing. A sequence-ready map must meet the following criteria. (1) It must be composed of clones that are efficient sequencing templates. (2) It must be assembled using a method that examines the entire cloned insert such as restriction digest fingerprinting. (3) The contig must have sufficient depth to allow the validation of the selected clones in the face of restriction pattern inconsistencies. In addition, increased contig depth will allow the selection of a more efficient minimal tiling path. A uniform depth of 6- to 10-fold coverage is recommended. (4) For large-scale projects, as large a contig as possible should be constructed in advance of selecting the minimal tiling path to avoid future closure problems. (NCI Thesaurus)

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Short (200 to 500 base pairs) DNA sequence that is operationally unique and has a single occurrence in the human genome and…

Sequence Specific Retention Factor

Predicts the retention time of tryptic peptides during RP-HPLC fractionation based on the hydrophobicity measure algorithm of Krokhin et al. (Mol Cell…

Sequence Similarity

The degree of similarity between the primary sequence of two biological macromolecules.

Sequence Object

An object representation of a gene sequence; provides access to the clones from which it was derived, the ASCII representation of the…

Sequence Number

A number that identifies the ordering relations (in time, space, etc.) in a set of objects or events.

Sequence Feature

An identifiable region or site of interest in a sequence.