Polyacrylamide Gel Electrophoresis

Definition 1

Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. (NCI Thesaurus)

Definition 2

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (NLM, Medical Subject Headings)

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